The purpose of Protecting Wetlands and Wildlife Habitat While Reducing Flood Losses: A Guidebook on Interagency Collaboration in the Mississippi River Basin is to illustrate the opportunities for wetland managers, hazard mitigation planners and other conservation and hazard mitigation professionals to work together to protect wetlands, water quality, and wildlife habitat and strengthen resilience to flooding in the Rock River Basin as well as the entire Upper Mississippi River Basin. The Guidebook provides a summary of the 2011 Wetlands, Wildlife Habitat, and Flood Hazards in the Rock River Basin Workshop, highlights key findings, and illustrates how to create maps that show where flood hazard areas and wetland and wildlife habitat areas overlap. It also identifies opportunities for facilitating interagency collaboration and includes brief case studies of successful interagency collaboration.